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Image Search Results


( A ) Average distribution of ATAC-seq signal around promoters (±3 kb from TSS) and enhancers in MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M (0.1 μM). ( B ) A volcano plot comparing Footprint TF activity between MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M. ( C ) Footprint TF activity of NFYA between MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M. ( D ) A volcano plot of DAPs between MCF7-ABER cells treated with Ctr-ASO-M and SUV-ASO2-M. ( E ) Category of DAPs according to their status (open or closed) and location (proximal or distal from TSS) in the genome. ( F ) Overlapping of up-regulated DEGs and open DAPs in MCF7-ABER cells treated with SUV-ASO2-M. The overlapped genes were analyzed using KEGG enrichment. ( G ) A heatmap of the overlapped genes enriched in the p53 pathway, senescence, or both processes. ( H ) IGV visualization of DAPs in p21 , GADD45A , and SERPINE1 of MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M.

Journal: Science Advances

Article Title: Targeting SUV39H2 exon skipping reverses resistance to CDK4/6 inhibitors via transcriptional activation of p21

doi: 10.1126/sciadv.aea2001

Figure Lengend Snippet: ( A ) Average distribution of ATAC-seq signal around promoters (±3 kb from TSS) and enhancers in MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M (0.1 μM). ( B ) A volcano plot comparing Footprint TF activity between MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M. ( C ) Footprint TF activity of NFYA between MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M. ( D ) A volcano plot of DAPs between MCF7-ABER cells treated with Ctr-ASO-M and SUV-ASO2-M. ( E ) Category of DAPs according to their status (open or closed) and location (proximal or distal from TSS) in the genome. ( F ) Overlapping of up-regulated DEGs and open DAPs in MCF7-ABER cells treated with SUV-ASO2-M. The overlapped genes were analyzed using KEGG enrichment. ( G ) A heatmap of the overlapped genes enriched in the p53 pathway, senescence, or both processes. ( H ) IGV visualization of DAPs in p21 , GADD45A , and SERPINE1 of MCF7-ABER cells treated with Ctr-ASO-M or SUV-ASO2-M.

Article Snippet: Antibodies against SUV39H2 (11338-1-AP), p21 (10355-1-AP), glyceraldehyde-3-phosphate dehydrogenase (60004-1-Ig), SERPINE1 (13801-1-AP), PLK2 (15956-1-AP), ESRP1 (21045-1-AP), UPF1 (23379-1-AP), anti-mouse (SA00001-1), and anti-rabbit secondary antibodies (SA00001-2) were products from Proteintech.

Techniques: Activity Assay

Pronounced PAI-1 signals are detectable in both A431 and Cal-39 VSCC cells. Detection of PAI-1 protein expression in three different passages of A431 and Cal-39 VSCC cells using western blot. PAI-1: Plasminogen Activator Inhibitor-1; VSCC: vulvar squamous cell carcinoma.

Journal: In Vivo

Article Title: Inhibition of Plasminogen Activator Inhibitor-1 (PAI-1) by Tiplaxtinin Attenuates the Aggressive Phenotype of Vulvar Squamous Cell Carcinoma Cells In Vitro

doi: 10.21873/invivo.14345

Figure Lengend Snippet: Pronounced PAI-1 signals are detectable in both A431 and Cal-39 VSCC cells. Detection of PAI-1 protein expression in three different passages of A431 and Cal-39 VSCC cells using western blot. PAI-1: Plasminogen Activator Inhibitor-1; VSCC: vulvar squamous cell carcinoma.

Article Snippet: The cells were treated with PAI-1 inhibitor tiplaxtinin (PAI-039, TPX) (MedChemExpress, Monmouth Junction, NJ, USA) dissolved in 2% v/v ethanol (Th.

Techniques: Expressing, Western Blot